🧬 Isom Lab Publications

  1. Lee K, Taylor S, Arcuri J, Chandthakuri S, Pujols J, Colon B, Wang Q, Wu C, Meng Z, Thompson-Ceccato S, Mitchell J, Bayik D, Carbone A, Slepak V, Slepak TI, Welford S, Ivan ME, Wang D, Goldberg B, Baringer W, Maradzike E, Isom DG. TM184C is a GPCR-like regulator of intercellular exchange and autophagy. Nature. (Accepted)
  2. Rostamian H, Madden EW, Kaplan FM, Kim RS, Isom DG, Strahl BD. Protocol for CRISPR genome editing in S. cerevisiae using PCR-based guide insertion. STAR Protoc. 2026 Jul 28;7(3):104751. Epub 2026 Jul 28. doi:10.1016/j.xpro.2026.104751 PMID: 42519825
  3. Wang D, Horton TM, Lee KD, Ogobuiro IC, Vatankhah F, de Andrade I, Pisano JC, Thakur VS, Gurevich MK, Taylor S, Alyamani M, Gannamedi DP, Isom DG, Lombard DB, Griswold AJ, Sharifi N, Tcheuyap VT, Brugarolas J, Gonzalgo ML, Kryvenko ON, Welford SM. Lactate-Activated GPR132 Signaling Drives a Tumor Microenvironmental Autocrine Metabolic Loop in Kidney Cancer. Cancer Res. 2026 Jun 6. Epub 2026 Jun 6. doi:10.1158/0008-5472.CAN-25-3236 PMID: 42258526
  4. Lee KD, Surana G, Taylor S, Isom DG. Proton-gating of opioid receptors and isoform functionality. bioRxiv. 2026 May 29. doi:10.64898/2026.05.26.727816
  5. Lee KD, Taylor S, Isom DG. Modulating proton sensing by the adenosine receptor A2. bioRxiv. 2026 May 29. doi:10.64898/2026.05.26.727808
  6. Taylor S, Colon B, Lee KD, Arcuri J, Chandthakuri S, Isom DG. Next-generation genetically encoded biosensors for spatiotemporal intracellular pH monitoring, mapping, and profiling. bioRxiv. 2026 May 21. doi:10.64898/2026.05.19.726343
  7. Pujols J, Lee KD, Kapolka NJ, Taylor S, Colon B, Isom DG. Systematic mapping of chimera-permissive sites by CRISPR-guided PAM scanning. JBC. (Accepted; preprint on bioRxiv.) doi:10.64898/2026.05.06.723292
  8. Si Y, Adhikari K, Herring LE, Isom DG, Li S, Lyons SP, McRitchie SL, Rushing BR, Sumner SJ, Dohlman HG. Nonredundant roles for paralogous proteins in the yeast glucose-sensing pathway. Mol Biol Cell. 2026 Feb 1;37(2):ar15. Epub 2025 Dec 16. doi:10.1091/mbc.E25-10-0475 PMID: 41400924
  9. Rowe JB, Lee K, Isom DG. PIONEER: A periplasmic display platform for synthetic biology-based screening of genetically encoded protein regulators. JBC. 2026 Jan;302(1):110967. Epub 2025 Nov 20. doi:10.1016/j.jbc.2025.110967 PMID: 41274507
  10. Kapolka NJ, Taghon GJ, Isom DG. Advances in yeast synthetic biology for human G protein-coupled receptor biology and pharmacology. Curr Opin Biotechnol. 2024 Aug;88:103176. Epub 2024 Jul 29. doi:10.1016/j.copbio.2024.103176 PMID: 39079313
  11. Wang D, Mahmud I, Thakur VS, Tan SK, Isom DG, Lombard DB, Gonzalgo ML, Kryvenko ON, Lorenzi PL, Tcheuyap VT, Brugarolas J, Welford SM. GPR1 and CMKLR1 Control Lipid Metabolism to Support the Development of Clear Cell Renal Cell Carcinoma. Cancer Res. 2024 Jul 2;84(13):2141-2154. doi:10.1158/0008-5472.CAN-23-2926 PMID: 38640229
  12. Morales Rodríguez LM, Crilly SE, Rowe JB, Isom DG, Puthenveedu MA. Location-biased activation of the proton-sensor GPR65 is uncoupled from receptor trafficking. PNAS. 2023 Sep 26;120(39):e2302823120. Epub 2023 Sep 18. doi:10.1073/pnas.2302823120 PMID: 37722051
  13. Kapolka NJ, Rowe JB, Taghon GJ, Morgan WM, O'Shea CR, Isom DG. Proton-gated coincidence detection is a common feature of GPCR signaling. PNAS. 2021 Jul 13;118(28):e2100171118. Epub 2021 Jul 6. doi:10.1073/pnas.2100171118 PMID: 34260394
  14. Taghon GJ, Rowe JB, Kapolka NJ, Isom DG. Predictable cholesterol binding sites in GPCRs lack consensus motifs. Structure. 2021 May 6;29(5):499-506.e3. Epub 2021 Jan 27. doi:10.1016/j.str.2021.01.004 PMID: 33508215
  15. Vilar S, Isom DG. One Year of SARS-CoV-2: How Much Has the Virus Changed?. Biology (Basel). 2021 Jan 26;10(2):91. Epub 2021 Jan 26. doi:10.3390/biology10020091 PMID: 33530355
  16. Rowe JB, Kapolka NJ, Taghon GJ, Morgan WM, Isom DG. The evolution and mechanism of GPCR proton sensing. JBC. 2021;296:100167. Epub 2020 Dec 13. doi:10.1074/jbc.RA120.016352 PMID: 33478938
  17. Kapolka NJ, Isom DG. HCAR3: an underexplored metabolite sensor. Nat Rev Drug Discov. 2020 Nov;19(11):745. doi:10.1038/d41573-020-00173-2 PMID: 33009526
  18. Luna LA, Lesecq Z, White KA, Hoang A, Scott DA, Zagnitko O, Bobkov AA, Barber DL, Schiffer JM, Isom DG, Sohl CD. An acidic residue buried in the dimer interface of isocitrate dehydrogenase 1 (IDH1) helps regulate catalysis and pH sensitivity. Biochem J. 2020 Aug 28;477(16):2999-3018. doi:10.1042/BCJ20200311 PMID: 32729927
  19. Rowe JB, Taghon GJ, Kapolka NJ, Morgan WM, Isom DG. CRISPR-addressable yeast strains with applications in human G protein-coupled receptor profiling and synthetic biology. JBC. 2020 Jun 12;295(24):8262-8271. Epub 2020 May 1. doi:10.1074/jbc.RA120.013066 PMID: 32358068
  20. Kapolka NJ, Taghon GJ, Rowe JB, Morgan WM, Enten JF, Lambert NA, Isom DG. DCyFIR: a high-throughput CRISPR platform for multiplexed G protein-coupled receptor profiling and ligand discovery. PNAS. 2020 Jun 9;117(23):13117-13126. Epub 2020 May 20. doi:10.1073/pnas.2000430117 PMID: 32434907
  21. Jiang C, Moorthy BT, Patel DM, Kumar A, Morgan WM, Alfonso B, Huang J, Lampidis TJ, Isom DG, Barrientos A, Fontanesi F, Zhang F. Regulation of Mitochondrial Respiratory Chain Complex Levels, Organization, and Function by Arginyltransferase 1. Front Cell Dev Biol. 2020;8:603688. Epub 2020 Dec 21. doi:10.3389/fcell.2020.603688 PMID: 33409279
  22. Lassuthova P, Rebelo AP, Ravenscroft G, Lamont PJ, Davis MR, Manganelli F, Feely SM, Bacon C, Brožková DŠ, Haberlova J, Mazanec R, Tao F, Saghira C, Abreu L, Courel S, Powell E, Buglo E, Bis DM, Baxter MF, Ong RW, Marns L, Lee YC, Bai Y, Isom DG, Barro-Soria R, Chung KW, Scherer SS, Larsson HP, Laing NG, Choi BO, Seeman P, Shy ME, Santoro L, Zuchner S. Mutations in ATP1A1 Cause Dominant Charcot-Marie-Tooth Type 2. Am J Hum Genet. 2018 Mar 1;102(3):505-514. doi:10.1016/j.ajhg.2018.01.023 PMID: 29499166
  23. Isom DG, Page SC, Collins LB, Kapolka NJ, Taghon GJ, Dohlman HG. Coordinated regulation of intracellular pH by two glucose-sensing pathways in yeast. JBC. 2018 Feb 16;293(7):2318-2329. Epub 2017 Dec 28. doi:10.1074/jbc.RA117.000422 PMID: 29284676
  24. Shellhammer JP, Morin-Kensicki E, Matson JP, Yin G, Isom DG, Campbell SL, Mohney RP, Dohlman HG. Amino acid metabolites that regulate G protein signaling during osmotic stress. PLoS Genet. 2017 May;13(5):e1006829. Epub 2017 May 30. doi:10.1371/journal.pgen.1006829 PMID: 28558063
  25. Isom DG, Sridharan V, Dohlman HG. Regulation of Ras Paralog Thermostability by Networks of Buried Ionizable Groups. Biochemistry. 2016 Jan 26;55(3):534-42. Epub 2016 Jan 6. doi:10.1021/acs.biochem.5b00901 PMID: 26701741
  26. Isom DG, Dohlman HG. Buried ionizable networks are an ancient hallmark of G protein-coupled receptor activation. PNAS. 2015 May 5;112(18):5702-7. Epub 2015 Apr 20. doi:10.1073/pnas.1417888112 PMID: 25902551
  27. Baker R, Wilkerson EM, Sumita K, Isom DG, Sasaki AT, Dohlman HG, Campbell SL. Differences in the regulation of K-Ras and H-Ras isoforms by monoubiquitination. JBC. 2013 Dec 27;288(52):36856-62. Epub 2013 Nov 18. doi:10.1074/jbc.C113.525691 PMID: 24247240
  28. Isom DG, Sridharan V, Baker R, Clement ST, Smalley DM, Dohlman HG. Protons as second messenger regulators of G protein signaling. Mol Cell. 2013 Aug 22;51(4):531-8. Epub 2013 Aug 15. doi:10.1016/j.molcel.2013.07.012 PMID: 23954348
  29. Isom DG, Marguet PR, Oas TG, Hellinga HW. A miniaturized technique for assessing protein thermodynamics and function using fast determination of quantitative cysteine reactivity. Proteins. 2011 Apr;79(4):1034-47. Epub 2011 Jan 5. doi:10.1002/prot.22932 PMID: 21387407
  30. Isom DG, Castañeda CA, Cannon BR, García-Moreno B. Large shifts in pKa values of lysine residues buried inside a protein. PNAS. 2011 Mar 29;108(13):5260-5. Epub 2011 Mar 9. doi:10.1073/pnas.1010750108 PMID: 21389271
  31. Isom DG, Castañeda CA, Cannon BR, Velu PD, García-Moreno E B. Charges in the hydrophobic interior of proteins. PNAS. 2010 Sep 14;107(37):16096-100. Epub 2010 Aug 26. doi:10.1073/pnas.1004213107 PMID: 20798341
  32. Isom DG, Vardy E, Oas TG, Hellinga HW. Picomole-scale characterization of protein stability and function by quantitative cysteine reactivity. PNAS. 2010 Mar 16;107(11):4908-13. Epub 2010 Mar 1. doi:10.1073/pnas.0910421107 PMID: 20194783
  33. Harms MJ, Castañeda CA, Schlessman JL, Sue GR, Isom DG, Cannon BR, García-Moreno E B. The pK(a) values of acidic and basic residues buried at the same internal location in a protein are governed by different factors. J Mol Biol. 2009 May 29;389(1):34-47. Epub 2009 Mar 24. doi:10.1016/j.jmb.2009.03.039 PMID: 19324049
  34. Isom DG, Cannon BR, Castañeda CA, Robinson A, García-Moreno B. High tolerance for ionizable residues in the hydrophobic interior of proteins. PNAS. 2008 Nov 18;105(46):17784-8. Epub 2008 Nov 12. doi:10.1073/pnas.0805113105 PMID: 19004768
  35. Mitra L, Hata K, Kono R, Maeno A, Isom DG, Rouget JB, Winter R, Akasaka K, García-Moreno B, Royer CA. V(i)-value analysis: a pressure-based method for mapping the folding transition state ensemble of proteins. J Am Chem Soc. 2007 Nov 21;129(46):14108-9. Epub 2007 Oct 26. doi:10.1021/ja073576y PMID: 17960902
  36. Brun L, Isom DG, Velu P, García-Moreno B, Royer CA. Hydration of the folding transition state ensemble of a protein. Biochemistry. 2006 Mar 21;45(11):3473-80. doi:10.1021/bi052638z PMID: 16533028
  37. Gong H, Isom DG, Srinivasan R, Rose GD. Local secondary structure content predicts folding rates for simple, two-state proteins. J Mol Biol. 2003 Apr 11;327(5):1149-54. doi:10.1016/s0022-2836(03)00211-0 PMID: 12662937