vesicle_colocalization_quantifier — Example input

This folder holds an example two-channel fluorescence microscopy field used to exercise the full vesicle_colocalization_quantifier pipeline end-to-end. The tool expects one maximum-projection TIFF per fluorophore channel; it thresholds each channel independently, detects contours with OpenCV, builds boolean masks, and computes their pixel-level intersection to quantify co-localized vesicles. A pre-made two-channel overlay is also included so the detected overlap regions can be visualized on the composite image. The outputs generated by running vesicle_colocalization_quantifier on these inputs live in ../example_output/.

• 📄 Maximum Projection_ch01_SV.tif – Download
• 📄 Maximum Projection_ch02_SV.tif – Download
• 📄 Maximum Projection_overlay.tif – Download

📜 Files in this folder

• Maximum Projection_ch01_SV.tif — Channel-01 maximum-projection TIFF: the fluorophore channel for vesicle population #1. Loaded into the “Channel 1 Image” slot in the GUI, then thresholded and contoured independently to produce the ch1 boolean mask that enters the overlap calculation.
• Maximum Projection_ch02_SV.tif — Channel-02 maximum-projection TIFF: the fluorophore channel for vesicle population #2, loaded into the “Channel 2 Image” slot and processed with its own threshold / contour settings. The pixel-level AND of the ch1 and ch2 masks is what defines the co-localized regions.
• Maximum Projection_overlay.tif — Pre-made two-channel overlay image, retained alongside the per-channel TIFFs for visual QA. Useful for eyeballing where the two populations coexist before the tool quantifies the overlap.