vesicle_triangulator β Example input
This folder holds an example two-channel fluorescence microscopy field that exercises the full vesicle_triangulator pipeline end-to-end. The tool expects per-channel maximum-projection TIFFs (one per fluorophore) plus a pre-made overlay of the two channels; it uses the overlay for the on-screen backdrop and the per-channel TIFFs for independent thresholding, component detection, and point extraction before triangulating across both channels. The outputs generated by running vesicle_triangulator on these inputs live in ../example_output/.
- π Maximum Projection_ch01_SV.tif β Download
- π Maximum Projection_ch02_SV.tif β Download
- π Maximum Projection_overlay.tif β Download
π Files in this folder
Maximum Projection_ch01_SV.tifβ Channel-01 maximum-projection TIFF: the fluorophore channel for vesicle population #1. Fed to the βThreshold Ch1β step to produce the binary mask, connected-component labeling, and point centroids that become one half of the triangulation.Maximum Projection_ch02_SV.tifβ Channel-02 maximum-projection TIFF: the fluorophore channel for vesicle population #2, processed independently by the βThreshold Ch2β step and contributing the complementary set of points.Maximum Projection_overlay.tifβ Combined two-channel overlay image used as the on-screen backdrop in the GUI and as the background raster on which the final triangulation, same-channel edges, and mixed (cross-channel) edges are drawn in the output PNGs.